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Calculate GC%, AT%, base counts, and a basic melting temperature for a DNA sequence.
Research & educational use only. These tools may produce mistakes — always verify results independently before relying on them. Use is at your own responsibility, and please don't enter real, sensitive, or clinical/patient data.
Sequence
Composition
Wallace rule Tm = 2°C·(A+T) + 4°C·(G+C); a quick estimate best suited to primers under ~14 nt.